MHC II DR (clone EBS-O-110), anti-human

Source:
A C3H mouse was immunized with Human PBL and HLA-homozygous B-LCL line.
Fusion partner: Sp2/0.

Specifications:
MHC class II molecules are encoded by polymorphic MHC genes and consist of a non-covalent complex of an αand βchain. Helper T lymphocytes bind antigenic peptides presented byMHC class II molecules. MHC class II molecules bind 13-18 amino acid antigenic peptides. Accumulating in endosomal/lysosomal compartments and on the surface of B cells, HLA-DM and -DO molecules regulate binding of exogenous peptides to class II molecules (HLA-DR) by sustaining a conformation that favors peptide exchange. The differential structural properties of MHC class I and class II molecules account for their respective roles in activating different populations of T lymphocytes.

Species reactivity:
Positive: human.

Applications:
Demonstration of MHC II DR.
Flow cytometry: +
Frozen sections<: +br />Immunofluorescence: +
Paraffin sections: ⎼

Positive control: Human PBL.

References:
- Sparrow RL, et al., Transplantation42: 647-652 (1986).
- Chorvath B et al. Neoplasma34(4): 417-425 (1987).
- Horejsi V et al. Tissue Antigens32(1): 6-11 (1988).
- Polakova K et al. Neoplasma 32(6): 641-8 (1985)