Midline-1 polyclonal, anti-human, mouse, rat

Midline-1 polyclonal, anti-human, mouse, rat

€388.00
In stock
SKU
BS8816
Catalog Number: BS8816
Size: 50 ul, 100 ul
Isotype: rabbit IgG
Applications: WB, IF

Datasheet
Background:
Midline-1 (Tripartite motif-containing protein 18, Putative transcription factor XPRF, RING finger protein 59) is a 667 amino acid protein encoded by the human gene MID1. Midline-1 belongs to the TRIM/RBCC family and contains two B box-type zinc fingers, one B30.2/SPRY domain, one COS domain, one fibronectin type-III domain and one RING-type zinc finger. Midline-1 is believed to have E3 ubiquitin ligase activity which targets the catalytic subunit of protein phosphatase 2 for degradation. It is a cytoplasmic protein found as a homodimer or heterodimer with Midline-2. It also interacts with IGBP1 (Lymphocyte signaling protein A4). Defects in MID1 are the cause of Opitz syndrome type I (OS-I). OS-I is an X-linked recessive disorder characterized by hypertelorism, genital-urinary defects such as hypospadias in males and splayed labia in females, lip-palate-laryngotracheal clefts, imperforate anus, developmental delay and congenital heart defects.

Alternative Name:
E3 ubiquitin-protein ligase Midline-1, Midin, Putative transcription factor XPRF, RING finger protein 59, RING finger protein Midline-1, Tripartite motif-containing protein 18, MID1, FXY, RNF59, TRIM18, XPRF

Application Dilution: WB: 1:500 - 1:2000, IF: 1:50 - 1:100

Specificity: Midline-1 polyclonal antibody detects endogenous levels of Midline-1 protein.

Immunogen:
Recombinant full length Human Midline-1.

MW: ~ 75kDa

Swis Prot.: O15344

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.
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