Monoclonal Anti-GST Tag

Background:
The glutathione S-transferase (GST, previously known as ligandins) family of enzymes are composed of many cytosolic, mitochondrial, and microsomal (now designated as MAPEG) proteins. GSTs are present in eukaryotes and in prokaryotes, where they catalyze a variety of reactions and accept endogenous and xenobiotic substrates. GST is commonly used to create fusion proteins. The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. A GST-tag is often used to separate and purify proteins that contain the GST-fusion. GST-fusion proteins can be produced in Escherichia coli, as recombinant proteins.

Immunogen:
Recombinant GST protein with full length.

Specificity: Recognize GST and GST fusion proteins.Western Blot (1:5000); ELISA (1:2000);

Product Form: Liquid

Storage Instruction:
Store at -20℃ for 1 year. Or aliquot and store at -80℃ for long term. Avoid repeated freeze and thaw cycles.