MRSA (MREJ, mec A, mec C) direct PCR Kit CE-IVDR

The MRSA direct PCR test is designed to detect MRSA directly from the swab on a commercially available qPCR cycler. It consists of a test kit with solutions that contain all necessary chemicals (buffer components, substances for PCR including an internal process control). After adding untreated patient sample to the reaction solution, the PCR analysis is carried out directly in the qPCR device.
The MRSA direct PCR test contains probes specific for the following targets: (1) MREJ, (2) mecA, (3) mecC, and (4) Internal Control. The probes are each labelled with fluorescent reporter dyes and a second dye that serves as a quencher and suppresses the fluorescence signals of the intact probes. The analysis is performed by determining Ct (cycle threshold) values. The Ct value describes the cycle in which the signal rises above a certain threshold for the first time. The more target copies (here: bacterial DNA) are present in the sample, the lower the Ct value. The Internal Control (IC) allows the detection of PCR inhibition. The buffer contains additives for bacterial lysis and enhancement of PCR efficiency. Patient samples can be analysed directly without prior DNA extraction.

Features
- Analyte(s): MREJ, mecA, mecC
- Sample origin: Human
- Sample type: nasal swab
- Assay type: qPCR (Realtime PCR)
- qPCR cycler detection channels: FAM/HEX/Red610/Cy5
- Quantity: 96 reactions/kit
- Certification: EU-IVDR