OGDH polyclonal, anti-human, mouse, rat

OGDH polyclonal, anti-human, mouse, rat

€388.00
In stock
SKU
BS8180
Catalog Number: BS8180
Size: 50 ul, 100 ul
Isotype: rabbit IgG
Applications: WB, IHC, IF

Datasheet
Background:
The α-ketoglutarate dehydrogenase (α-KGD) complex is a multienzyme complex which localizes to the mitochondrial matrix and consists of three protein subunits: α-ketoglutarate dehydrogenase, also designated α-KGD, E1k or oxoglutarate dehydrogenase (OGDH); dihydrolipoyl succinyltransferase (E2k or DLST); and dihydrolipoyl dehydrogenase (E3). The α-KGD subunit of the α-KGD complex catalyzes the conversion of α-ketoglutarate to succinyl-CoA and CO2, an essential reaction of the tricarboxylic acid cycle. A definciency in α-KGD results in hypotonia, metabolic acidosis, hyperlactatemia immediately after birth, and neurologic deterioration resulting in death at about 30 months of age. Low molar ratios of ketone bodies in plasma of neonates with congenital lactic acidosis are proposed indicators of tricarboxylic acid cycle dysfunction.

Alternative Name:
2-oxoglutarate dehydrogenase, mitochondrial, 2-oxoglutarate dehydrogenase complex component E1, OGDC-E1, Alpha-ketoglutarate dehydrogenase, OGDH

Application Dilution: WB: 1:500 - 1:2000, IHC: 1:50 - 1:200, IF: 1:50 - 1:200

Specificity: OGDH polyclonal antibody detects endogenous levels of OGDH protein.

Immunogen:
Recombinant full length Human OGDH.

MW: ~ 116 kDa

Swis Prot.: Q02218

Purification & Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Format:
1mg/ml in PBS with 0.1% Sodium Azide, 50% Glycerol.

Storage:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

For research use only, not for use in diagnostic procedure.
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