PIGF-2 human recombinant protein

Background:
PIGF-2, as know as placenta growth factor, is a member of the VEGF sub-family that a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. It is ultimately associated with angiogenesis. Specifically, it plays a role in trophoblast growth and differentiation. Also it is a potential biomarker for preeclampsia, a condition in which blood vessels in the placenta are too narrow, resulting in high blood pressure. Under normal physiologic conditions, it is also expressed at a low level in other organs including the heart, lung, thyroid, and skeletal muscle. Recombinant human PIGF-2, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Alternative Names:
placental growth factor, placenta growth factor isoform 1, PGF, D12S1900, PGFL, PIGF, PLGF, PlGF-2, SHGC-10760

Domain: 19-170aa
Sequence:
LPAVPPQQWA LSAGNGSSEV EVVPFQEVWG RSYCRALERL VDVVSEYPSE VEHMFSPSCV SLLRCTGCCG DENLHCVPVE TANVTMQLLK IRSGDRPSYV ELTFSQHVRC ECRPLREKMK PERRRPKGRG KRRREKQRPT DCHLCGDAVP RR

UniProt No.: P49763-3
NCBI Accession No.: NP_002623.2

Molecular Weight: 18.1kDa (158aa)
Concentration: 0.5mg/ml (determined by Absorbance at 280nm)
Formulation: Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Purity: > 90% by SDS - PAGE
Bioactivity: Measured by its binding ability in a functional ELISA with Human VEGFR1/Flt-1 (CAT# ATGP4101).
Endotoxin: < 1 EU per 1ug of protein (determined by LAL method)
Endotoxin: His-Tag
Storage: Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.