PRImeDETECT™ Salmonella Detection Kit

PRImeDETECT™ Salmonella Detection Kit (Salmonella spp) is an ideal tool for a fast and reliable Amplification and Detection of specific DNA fragments from Salmonella spp by the real-time PCR method.

The Kit includes all reagents required in a comfortable ready-to-use Multiplex PCR MasterMix. The optimized MasterMix contains a Buffer, dNTPs, Hot-start DNA Polymerase, DNA-free water, MgCl2 and an Internal Amplification Control (IAC) whose detection indicates the absence of PCR inhibitors.

Primers and Probes for the amplification of IAC as well as for the amplification of the target gene are included in the MasterMix. The Probe for the detection of target gene is labelled with the FAM, whereas the probe for the detection of IAC is labelled with the JOE fluorochrome.

In addition, the kit includes both Positive Control and Negative Control. The Positive control is supplied to demonstrate that the PCR amplification is working efficiently with the supplied components. To confirm absence of contamination, a Negative control reaction should be included every time the kit is used. Include DNAready Lysis Buffer to extract the DNA from the sample prior to PCR Detection.

Advantages & Features
- Easy and Ready-to-use Kit.
- Fast: results in less than 24 hours with minimal handling.
- Reliable and highly specific Results.
- High Quality: manufactured according strict quality controls in every manufacturing step to guarantee the highest reproducibility.
- Customizable Multiplex Solutions: according to your requirements, to measure up to 5 Pathogens of your choice at the same time in 1 kit.

Specifications
- Amplification limit: 10 UG per reaction (100%), 2 UG per reaction (40%).
- Quantification limit: 20 UG per reaction (100%).
- Quantification Dynamic range: 6 logs.
- Target: Salmonella spp.
- Inclusivity: 100% Inclusivity. 700 Salmonella strains tested, including S.enterica and S. bongori
- Exclusivity: 100%. Tested with 42 strains of the rest of Enterobacteriaceae and Eubacteria
- Detection:Probe labelled with fluorescent dyes Salmonella spp: FAM-BHQ1; IAC: HEX-BHQ1.
- Thermal cCycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers.

Includes
- 1 x PCR MasterMix
- 1 x DNAready Lysis Buffer
- 1 x PCR Positive Control
- 1 x PCR Negative Control

Shipping & Storage
Shipment: at Ambient Temperature.
Storage: -20 °C upon receipt. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month.