Actin (C-terminal region) (clone M202), anti-human, mouse, rat, chicken
€355.00
In stock
SKU
ECM-AM2021
Catalog Number: ECM-AM2021
Size: 100 μl
Isotype: mouse IgG2a
Applications: WB, E, ICC, IHC
Reactivity: Hu, Ms, Rt, Ck
Datasheet
Questions? Contact us!
Size: 100 μl
Isotype: mouse IgG2a
Applications: WB, E, ICC, IHC
Reactivity: Hu, Ms, Rt, Ck
Datasheet
Questions? Contact us!
Background:
Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four α isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (β and γ). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.
Immunogen: Clone (M202) was generated from a sequence corresponding to amino acids in the C-terminal region of human b-actin. This human actin sequence is highly conserved in most eukaryotic actin isoforms.
Specificity: This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of human A431, SYF, and HUVEC cells, as well as mouse C2C12 cells.
Application dilution:
ELISA: 1:2000
ICC: 1:50
IHC: 1:50
WB: 1:1000
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Mouse monoclonal, protein G purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
Product References:
Dutta, P et al. (2014) J Neurochem. 130(3):360 WB: rat brain
Muirhead, G et al. (2014) J Mol Neurosci. 53(1):125 WB: rat brain
Pritchard, AJ et al. (2014) PLoS One. 9(6):e99444 WB: mouse splenocytes
Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four α isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (β and γ). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.
Immunogen: Clone (M202) was generated from a sequence corresponding to amino acids in the C-terminal region of human b-actin. This human actin sequence is highly conserved in most eukaryotic actin isoforms.
Specificity: This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of human A431, SYF, and HUVEC cells, as well as mouse C2C12 cells.
Application dilution:
ELISA: 1:2000
ICC: 1:50
IHC: 1:50
WB: 1:1000
End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
Buffer/Storage:
Mouse monoclonal, protein G purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at -20°C. Stable for 1 year.
Product References:
Dutta, P et al. (2014) J Neurochem. 130(3):360 WB: rat brain
Muirhead, G et al. (2014) J Mol Neurosci. 53(1):125 WB: rat brain
Pritchard, AJ et al. (2014) PLoS One. 9(6):e99444 WB: mouse splenocytes
| Is Featured? | No |
|---|
Write Your Own Review