TNF-alpha human active recombinant protein

Protein Family: Chaperones and Folding Catalysts, CAM Ligands, Cytokines

Pathway and Disease: Immune System, Cell Growth and Death, Signal Transduction, Infectious Diseases, Signaling Molecules and Interaction, Cell Growth and Death, Endocrine System, Neurodegenerative Disorders

Alternate Names: TNF-alpha, Tumour necrosis factor alpha, TNFA, TNF-alpha, Tumor necrosis factor ligand superfamily 2, TNFSF2, Cachectin, Cachexin, APC1 protein, DIFDescription:
Tumour necrosis factor alpha (TNF alpha) is a potent pleiotrophic cytokine produced by white blood cells, endothelium, and several other normal and tumour cells in response to a wide variety of stimuli. TNF alpha is involved in the pathophysiological processes of several chronic and acute diseases, including the induction of apoptosis and up regulation of inflammation. Recombinant TNF alpha comprises a 157 amino acid fragment (77-233) corresponding to the mature soluble TNF alpha chain protein. TNF alpha is expressed in E. coli with an amino-terminal hexahistidine tag.

Source: E. coli

Applications: E, WB

Accession No.: P01375

MW: 21.85 kDa

Sequence:
His-Tag sequence not included: VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL

Purity:
Purity > 95% as determined by reducing and non-reducing SDS-PAGE, Bradford assay, Western blot and ELISA.

Format:
Each vial contains 0.01 mg protein (liquid) in PBS pH7.4, 50% glycerol. Final concentration 0.1-1 mg/ml.

Storage: Store at -20°C. Product is guaranteed one year from the date of shipment.