ToBoScanTM -More accurate detection and definition of blood monocyte & TiMa subset
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You are enquiring about ToBoScanTM -More accurate detection and definition of blood monocyte & TiMa subset
ToBoScan:
Single-use tubes containing the following fluorochrome-conjugated antibodies in an optimized dried formulation, including backbone markers for the screening of different stages of monocytes and macrophages in blood.
Both FlowBoScan and ToBoScan solutions incorporate the most informative markers, ensuring precise results with minimal effort. Specifically designed for macrophage detection, they offer a minimally invasive approach for accurate analysis. Beyond their primary function, the tools’ versatility extends to potential applications in cancer screening and tissue homeostasis studies, making them an indispensable asset for researchers exploring diverse aspects of biological systems.
ToBoScan consists of single-use tubes containing the following fluorochrome conjugated antibodies in an optimized dried formulation, including backbone markers for the screening of different stages o fmonocytes and macrophages in blood.
-HLA-DR CF-Blue, CD45 OC515, CD64 FITC, CD14 PerCP-Cyanine5.5, CD300E PE-Cyanine7, CD16 APC-C750
Advantages:
- Easy, fast, reliable and disruptive tool
- Most informative and relevant markers included
- Single use tube avoiding errors
- Dried combinaiton ready-to-use and extended stability
- Minimally invasive tool for macrophage detection
- Potential applications in cancer screening and tissue homeostasis studies
Characteristics of blood monocytes and tissue macrophages:
Monocytes are produced by the bone marrow from precursors. Monocytes circulate in the bloodstream for about one to three days and then typically migrate into tissues throughout the body where they differentiate into macrophages which have the function of phagocytosing bacteria and damaged tissue.
Therefore the vast majority of monocytes (90-95%) in human blood are CD14++/CD16-/dim “classical monocytes”, whereas macrophages in human tissues are generally CD14dim/CD16+/++. Interestingly, in human lymph most monocytes/macrophages (65-95% )have the “non-classical” CD14dim/CD16++ phenotype.This suggests that the small population (5-10%) of CD14dim/CD16++ “non-classical monocytes” in blood are most likely Tissue macrophages (TiMas), which have returned from their patrolling and scavenger tasks in the body tissues.
Different studies have identified changes in the absolute and relative numbers of circulating monocytes and TiMas in clinical conditions with significant tissue disruption, such as in case of inflammation, sepsis, autoimmune disease, and cancer.Therefore, acurate detection and definition of bloodmonocyte & TiMa subset represent anovel tool for early diagnosis and treatment monitoring in oncology and tissue homeostasis.
References
1. van den Bossche WBL, et al. Monocytes carrying GFAP detect glioma ,brain metastasis and ischaemic stroke, and predict glioblastoma survival. Brain Communications 2020; 3(1): fcaa215.
2. van den Bossche WBL, et al .Flow cytometric assessment of leukocyte kinetics for the monitoring of tissue damage. Clinical Immunology. 2018 Dec; 197:224-30.
3. Damasceno D,et al. Distribution of subsets of blood monocytic cells throughout life. 2019 Jul; 144(1): 320-3.e6.
4. Kapellos TS, et al. Human Monocyte Subsets and Phenotypes in Major Chronic Inflammatory Diseases. Frontiers in Immunology. 2019 Aug.10: 2035.
5. Talati, T, et all. Monocyte subset analys is accurately distinguishes CMML from MDS and is associated with a favorable MDS prognosis. Blood. 2017 Mar. 129(13): 1881-3.
6. Sampath P, et al. Monocyte Subsets: Phenotypes and Function in Tuberculosis Infection. Frontiersin Immunology. 2018 Jul.9:1726.
Single-use tubes containing the following fluorochrome-conjugated antibodies in an optimized dried formulation, including backbone markers for the screening of different stages of monocytes and macrophages in blood.
Both FlowBoScan and ToBoScan solutions incorporate the most informative markers, ensuring precise results with minimal effort. Specifically designed for macrophage detection, they offer a minimally invasive approach for accurate analysis. Beyond their primary function, the tools’ versatility extends to potential applications in cancer screening and tissue homeostasis studies, making them an indispensable asset for researchers exploring diverse aspects of biological systems.
ToBoScan consists of single-use tubes containing the following fluorochrome conjugated antibodies in an optimized dried formulation, including backbone markers for the screening of different stages o fmonocytes and macrophages in blood.
-HLA-DR CF-Blue, CD45 OC515, CD64 FITC, CD14 PerCP-Cyanine5.5, CD300E PE-Cyanine7, CD16 APC-C750
Advantages:
- Easy, fast, reliable and disruptive tool
- Most informative and relevant markers included
- Single use tube avoiding errors
- Dried combinaiton ready-to-use and extended stability
- Minimally invasive tool for macrophage detection
- Potential applications in cancer screening and tissue homeostasis studies
Characteristics of blood monocytes and tissue macrophages:
Monocytes are produced by the bone marrow from precursors. Monocytes circulate in the bloodstream for about one to three days and then typically migrate into tissues throughout the body where they differentiate into macrophages which have the function of phagocytosing bacteria and damaged tissue.
Therefore the vast majority of monocytes (90-95%) in human blood are CD14++/CD16-/dim “classical monocytes”, whereas macrophages in human tissues are generally CD14dim/CD16+/++. Interestingly, in human lymph most monocytes/macrophages (65-95% )have the “non-classical” CD14dim/CD16++ phenotype.This suggests that the small population (5-10%) of CD14dim/CD16++ “non-classical monocytes” in blood are most likely Tissue macrophages (TiMas), which have returned from their patrolling and scavenger tasks in the body tissues.
Different studies have identified changes in the absolute and relative numbers of circulating monocytes and TiMas in clinical conditions with significant tissue disruption, such as in case of inflammation, sepsis, autoimmune disease, and cancer.Therefore, acurate detection and definition of bloodmonocyte & TiMa subset represent anovel tool for early diagnosis and treatment monitoring in oncology and tissue homeostasis.
References
1. van den Bossche WBL, et al. Monocytes carrying GFAP detect glioma ,brain metastasis and ischaemic stroke, and predict glioblastoma survival. Brain Communications 2020; 3(1): fcaa215.
2. van den Bossche WBL, et al .Flow cytometric assessment of leukocyte kinetics for the monitoring of tissue damage. Clinical Immunology. 2018 Dec; 197:224-30.
3. Damasceno D,et al. Distribution of subsets of blood monocytic cells throughout life. 2019 Jul; 144(1): 320-3.e6.
4. Kapellos TS, et al. Human Monocyte Subsets and Phenotypes in Major Chronic Inflammatory Diseases. Frontiers in Immunology. 2019 Aug.10: 2035.
5. Talati, T, et all. Monocyte subset analys is accurately distinguishes CMML from MDS and is associated with a favorable MDS prognosis. Blood. 2017 Mar. 129(13): 1881-3.
6. Sampath P, et al. Monocyte Subsets: Phenotypes and Function in Tuberculosis Infection. Frontiersin Immunology. 2018 Jul.9:1726.
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